Abstract Background Engineered therapeutic cells have attracted a great deal of interest due to their potential applications in treating a wide range of diseases, including cancer and autoimmunity.Chimeric antigen receptor (CAR) T-cells are designed to detect and Bottom-up approach to strengthen community-based malaria control strategy from community health workers’ perceptions of their past, present, and future: a qualitative study in Palawan, Philippines kill tumor cells that present a specific, predefined antigen.The rapid expansion of targeted antigen beyond CD19, has highlighted new challenges, such as autoactivation and T-cell fratricide, that could impact the capacity to manufacture engineered CAR T-cells.Therefore, the development of strategies to control CAR expression at the surface of T-cells and their functions is under intense investigations.
Results Here, we report the development and evaluation of an off-switch directly embedded within a CAR construct (SWIFF-CAR).The incorporation of a self-cleaving degradation moiety controlled by a protease/protease inhibitor pair allowed the ex vivo tight and reversible control of the CAR surface presentation and the subsequent CAR-induced signaling Pantalla Itinerante: Cine, vino y espacio and cytolytic functions of the engineered T-cells using the cell permeable Asunaprevir (ASN) small molecule.Conclusions The strategy described in this study could, in principle, be broadly adapted to CAR T-cells development to circumvent some of the possible hurdle of CAR T-cell manufacturing.This system essentially creates a CAR T-cell with an integrated functional rheostat.